Effects of long-term tea polyphenols consumption on hepatic microsomal drug-metabolizing enzymes and

AIM: To investigate the effects of long-term tea polyphenols (TPs) consumption on hepatic microsomal drug-metabolizing enzymes and liver function in rats. METHODS: TPs were administered intragastrically to rots at the doses of 833 mg.kg.^-1d^-1 (n=20) and 83.3 mg.kg^-1.d^-1(n=20) respectively for six months. Controlled group (n=20) was given same volume of saline solution. Then the contents of cytochrome P450, b5, enzyme activities of aminopyrine N-demethylase (ADM), glutathione S-trasferase (GST) and the biochemical liver function of serum were determined. RESULTS: The contents of cytochrome P450 and bs in the livers of male rats in high dose groups (respedJvely 2.66±0.55,10.43±2.78 nmol.mg MS pro^-1) were significantly increased compared with the control group (1.08±1.04, 5.51±2.98nmol.mg MS pro^-1; P<0.01, respectively). The enzymatic activities of ADM in the livers of female rats in high dose groups (0.91±0.08 mmol.mg MS pro^-lmin^-1) were increased compared with the control group (0.82±0.08 mmol.mg MS pro^-l.min^-1; P<0.05). The GST activity was unchanged in all treated groups, and the function of liver was not obviously changed. CONCLUSION: The antidotal capability of rats" livers can be significantly improved after long-term consumption of TPs. There are differences in changes of drug-metabolizing enzymes between the sexes induced by TPs and normal condition.

完成机构：DepartmentofPharmacy,FirstAffiliatedHospital,GuangxiMedicalUniversity,Nanning530021,GuangxiZhuangAutonomousRegion,China